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Webinar:
Correlating Raman and fluorescence lifetime imaging
Thursday 23rd March - 2am CDT | 7am GMT | 8am CET | 3pm HKT (Hong Kong)
Note: This event has now finished, you can watch all of our webinars on demand on our webinars.
Register for our webinar:
Correlating Raman and fluorescence lifetime imaging
Thursday 23rd March - 2am CDT | 7am GMT | 8am CET | 3pm HKT (Hong Kong)
FLIM allows the molecular environment of biological cells and tissue to be studied. Changes in pH, ion concentration and molecular binding can be detected. FLIM is a fast technique, so it provides an effective precursor to highly specific chemical analysis via Raman imaging. FLIM is compatible with Raman spectroscopy as it utilises autofluorescence, so it can be used for label-free sample analysis.
Within this webinar, we will describe how we have integrated FLIM hardware, developed by Becker & Hickl, onto a Renishaw inVia Raman microscope. Both techniques employ the same optical arrangement and mapping stage hardware. This means that FLIM and Raman data can be collected from the same sample area with pixel-to-pixel accuracy.
We will explain how correlative FLIM/Raman can analyse different materials and can be used for initial identification of areas with interesting molecular interactions. Then, we explain how targeted Raman spectroscopy is used to gain an understanding of the underlying biochemistry.
Overall, we will demonstrate a solution for performing both Raman and FLIM on a single microscope system. This enables detailed physical and chemical information to be collected from samples with ease.
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Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book.
Heading 5
Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book.
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Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book.
Meet your experts:
Dale Boorman, Biology Applications Specialist
Dale is an expert in the biological applications of Raman spectroscopy, having completed a PhD in cell and molecular biology at Cardiff University. At Renishaw, Dale provides Raman solutions for cell- and tissue-based studies, helping to move the technique from the lab to the clinic.
Julius Heitz, Applications specialist at Becker & Hickl
Receiving a doctorate from the Free University of Berlin, Heitz studied spintronic effects in solid-state samples with high-field terahertz radiation sources, gaining a strong basis in nonlinear optics and femtosecond laser physics along the way. He is currently an applications specialist at Becker & Hickl GmbH, with a focus in research and development, and customer support, working with time-correlated single-photon counting.